解:∵Rt△BCD中,BC=4cm,BD=5cm,∴CD= BD2?BC2 = 52?42 =3cm,过D作DE⊥AB于E,∵BD是∠ABC的平分线,∠C=90°,DE⊥AB,∴DE=CD,∵CD=3cm,∴DE=3cm.故选C.